Patrick G. Needham

  • Research Assistant Professor

Contact

Office: (412) 624-4830
A321 Langley Hall
4249 Fifth Avenue
Pittsburgh, PA 15260

As part of the Pittsburgh Center for Kidney Research I have been invovled in the study of the biogenesis and quality control decisions of several clinically important ion channels. We investigate the factors that lead to folding or degradation of these proteins in the endoplasmic reticulum and have identified important chaperone proteins that regulate this process.

In a separate project I have been characterizing the biochemical properties of wild type and mutant forms of the yeast Hsp70 chaperone protein Ssa1. These studies will provide insight into the biochemical alterations of Ssa1 mutants and provide a better understanding of the Ssa1 activities that lead to altered phenotypes seen in yeast expressing the mutant protein.

Needham, P.G., H.J. Patel, G. Chiosis, P.H. Thi

Needham, P.G., H.J. Patel, G. Chiosis, P.H. Thibodeau, and J.L. Brodsky.  Mutations in the yeast Hsp70, Ssa1, at P417 alter ATP cycling, interdomain coupling, and specific chaperone functions. J. Mol. Biol. (2015) 427: 2948-2965

Buck, T.M., R. Jordan, J. Lyons-Weiler, J.L. Ad

Buck, T.M., R. Jordan, J. Lyons-Weiler, J.L. Adelman, P.G. Needham, T.R. Kleyman, and J.L. Brodsky. The expression of three topologically distinct membrane proteins elicits unique stress response pathways in the yeast Saccharomyces cerevisiae. Physiol. Genomics (2015) 47: 198-214

Surlow, B.A., B. Cooley, P.G. Needham, J.L. Bro

Surlow, B.A., B. Cooley, P.G. Needham, J.L. Brodsky, and J. Patton-Vogt.  Loss of Ypk1 accelerates Plb1-mediated phosphatidylcholine deacylation in S. cerevisiae.  J. Biol. Chem. (2014) 289(45):31591-604

Kolb, A. R., P. G. Needham, C. Rothenberg, C. J

Kolb, A. R., P. G. Needham, C. Rothenberg, C. J. Guerriero, P. A. Welling, and J. L. Brodsky.  ESCRT regulates surface expression of the Kir2.1 potassium channel.  Mol. Biol. Cell (2014) 25: 276- 289

Needham PG, Brodsky JL. How early studies on se

Needham PG, Brodsky JL. How early studies on secreted and membrane protein quality control gave rise to the ER associated degradation (ERAD) pathway: The early history of ERAD. Biochim Biophys Acta. (2013) 1883: 2447-2457

Donnelly BF, Needham PG, Snyder AC, Roy A, Khad

Donnelly BF, Needham PG, Snyder AC, Roy A, Khadem S, Brodsky JL, Subramanya AR. Hsp70 and Hsp90 Multichaperone Complexes Sequentially Regulate Thiazide-Sensitive Cotransporter ER-Associated Degradation and Biogenesis. J Biol Chem. (2013) 288: 13124-13135

Needham, P. G., K. Mikoluk, P. Dhakarwal, S. Kh

Needham, P. G., K. Mikoluk, P. Dhakarwal, S. Khadem, A. C. Snyder, A. R. Subramanya, and J. L. Brodsky. (2011)  The Thiazide-Sensitive NaCl Cotransporter is Targeted for Chaperone-Dependent ER-Associated Degradation. J. Biol. Chem. 286:43611-21

Botha, M., A.N. Chiang, P.G. Needham, L.L. Stephens, H.C. Hoppe, S. Kulzer, J.M. Przyborski, K. L

Botha, M., A.N. Chiang, P.G. Needham, L.L. Stephens, H.C. Hoppe, S. Kulzer, J.M. Przyborski, K. Lingelbach, P. Wipf, J.L. Brodsky, A. Shonhai, and G.L. Blatch (2011) Plasmodium falciparum encodes a single cytosolic type I Hsp40 that functionally interacts with Hsp70 and is upregulated by heat shock. Cell Stress Chaperones 16: 389-401

Needham PG, Masison DC. (2008) Prion-impairing

Needham PG, Masison DC. (2008) Prion-impairing mutations in Hsp70 chaperone Ssa1: effects on ATPase and chaperone activities. Arch Biochem Biophys. 478:167-74